Step-by-Step Examples is one of four manuals included with Prism 4. Enter final test articleconcentrations in the X column and corresponding percent maximal binding values in the Y column.1 Adapted from: Miller, J.R., GraphPad Prism Version 4.0 Step-by-Step Examples, GraphPad Software Inc., SanDiego CA, 2003. To keep the example simple, we’ll assume that binding measurements are made in singletand that data have already been normalized to percent of maximal binding (although you may use Prism tonormalize the data automatically-see the Step-by-Step Example “Analyzing Dose-Response Data” for details).In the Welcome dialog, indicate that you will choose the Format of data table and format the table forNumbers in the X column and A single column of values for each data set.On the data table, enter some example data describing a binding inhibition curve. The endpoint of your screen is the IC 50 of a curve fitted to the plot of agonist binding vs. You are evaluating entitiesfound active in a primary screen for their effect on binding of a standard agonist to an isolated receptorpreparation. The procedure in the following section is condensed from the Step-by-Step Example “AnalyzingDose-Response Data”.A Simple Project File for Computing IC 50Suppose that you are designing a secondary screen for a new-drug-discovery program.
Save the dose-response graph in a series of files, one for each test article.So that you will be able to use the script in this article, first prepare a Prism project file for evaluating bindinginhibition data. Save a list of IC 50 values to a single output text file, and3. Transfer dose-response data from a series of input text files to the Prism project file,2.
We’ll then create and use a Prism script to1. When new data are imported into the project file according to instructions in the script, the project file’sinternal links bring about reanalysis and regraphing automatically.In this article, we’ll create a Prism project file for graphing dose-response data and fitting a sigmoidal curve-ofbest-fitto that data. Version 4.0Step-by-Step ExamplesCreating and Running a Prism Script 1A Prism script is a set of instructions, written in a language unique to Prism and held in an ordinary text file, formoving raw data into, and output from, a Prism project file.Prism scripts do not contain instructions for analyzing and graphing data-those procedures inhere in the projectfile itself.
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